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Registros recuperados: 78
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A survey for maintenance of virulent newcastle disease virus-free area in poultry production in Brazil BJM
Orsi,M.A; Doretto Jr,L; Camillo,S.C.A; Reischak,D; Ribeiro,S.A.M; Ramazzoti,A; Mendonça,A.O; Spilki,F.R; Buzinaro,M.G; Ferreira,H.L; Arns,C.W.
In 2003, Brazil was recognized as a pathogenic Newcastle Disease Virus (NDV) strain-free country for commercial poultry. This research was conducted in Brazil between December 2003 and March 2005 to verify the maintenance of this virulent NDV-free status. Serum samples from 5,455 flocks for commercial poultry farms were collected, comprising 81,825 broiler chickens. The farms were located in nine states of the country, grouped in three geographic regions. Serological evidence of NDV infection was detected in 28.8% of the surveyed farms. However, all fifteen viruses isolated and identified as Newcastle Disease Virus (NDV) were characterized as nonpathogenic strains, based on the Intracerebral Pathogenicity Index. These results showed that Brazil preserves...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Newcastle Disease Virus; Pathogenicity; Poultry; Biological characterization.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000200017
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Aggressiveness between genetic groups I and II of isolates of Cercospora zeae-maydis Scientia Agricola
Mathioni,Sandra Marisa; Carvalho,; Brunelli,Kátia Regiane; Beló,André; Camargo,Luis Eduardo Aranha.
For many years, the gray leaf spot disease (GLS) caused by the fungus Cercospora zeae-maydis Tehon & Daniels, was not considered an important pathogen of maize (Zea mays, L.) in Brazil. However, the recent adoption of agronomical practices such as no-tillage and cultivation under central pivot irrigation systems increased the incidence and severity to the extent that GLS is now one of the most important diseases of maize. Isolates of C. zeae-maydis can be distinguished by two genetic groups (I and II) based on AFLP markers and on polymorphisms of the ITS and 5.8S rDNA regions. Until now, however, the biological implications of this distinction remain unclear. This study investigated whether isolates from the two genetic groups differ in...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Disease resistance; Gray leaf spot; Genetic variability; Pathogenicity.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162006000600006
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Amplification of the cap20 pathogenicity gene and genetic characterization using different markers molecular in Colletotrichum gloeosporioides isolates BABT
Maciel,Danielli Barreto; Medeiros,Lílian Vieira de; Medeiros,Vivian Vieira de; Leão,Mariele Porto Carneiro; Camargo,Luis Eduardo Aranha; Oliveira,Neiva Tinti de.
Studies were performed to analyze the genetic characterization using RFLP-ITS and Intron (primer EI1) markers and the amplification of the cap20 pathogenicity gene by PCR in Colletotrichum gloeosporioides isolates of different hosts plant. The genetic variability was accessed using RFLP-ITS and Intron markers and grouping by UPGMA method. Primers to cap20 gene were constructed using selected sequences of the GenBank (National Center of Biotechnology Information, http://www.ncbi.nlm.nih.gov) with the Primer 3 program. The dendrograms analysis showed that the RFLP-ITS marker was more informative to separate the Colletotrichum sp, and that primer EI1 demonstrated greater genetic diversity. The amplification of the DNA of the Colletotrichum isolates to the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cap20 gene; Colletotrichum gloeosporioides; Pathogenicity.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000600001
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An investigation of virulence factors of Legionella pneumophila environmental isolates BJM
Arslan-Aydoğdu,Elif Özlem; Kimiran,Ayten.
ABSTRACT Nine Legionella pneumophila strains isolated from cooling towers and a standard strain (L. pneumophila serogroup 1, ATCC 33152, Philadelphia 1) were analyzed and compared in terms of motility, flagella structure, ability to form biofilms, enzymatic activities (hemolysin, nucleases, protease, phospholipase A, phospholipase C, acid phosphatase, alkaline phosphatase and lipase), hemagglutination capabilities, and pathogenicity in various host cells (Acanthamoeba castellanii ATCC 30234, mouse peritoneal macrophages and human peripheral monocytes). All the isolates of bacteria appeared to be motile and polar-flagellated and possessed the type-IV fimbria. Upon the evaluation of virulence factors, isolate 4 was found to be the most pathogenic strain,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Legionella pneumophila; Pathogenicity; Virulence of environmental isolates.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000100189
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Análise de patogenicidade de isolados de Chrysodeixis includens NPV para uso na produção de bioinseticidas. Infoteca-e
SANTOS, L. A. V. M.; RIBEIRO, Z. M. de A.; FERNANDES, L. S. P.; FERREIRA, A. C. de Q.; MOTA, I. de S.; GOMES, A. C. M. M.; CRAVEIRO, S. R.; CASTRO, M. E. B. de.
Chrysodeixis includens (Walker, 1858) (Lepidoptera: Noctuidae) tem sido considerada uma praga severa em lavouras como a soja e o algodão, espécies de grande importância econômica para o Brasil. Inseticidas à base de baculovírus, um grupo de vírus patogênicos a insetos, têm sido usados com sucesso para controlar lepidópteros-praga na agricultura brasileira. A multiplicação em massa do vírus para a produção comercial deve partir da seleção de isolados virais com alta virulência para resultar em um produto com alta efetividade contra a praga no campo. Dois isolados virais, obtidos de lagartas infectadas de Chrysodeixis includens em plantações de soja em Buritis-MG, foram purificados, identificados, analisados e avaliados quanto à atividade biológica por meio...
Tipo: Boletim de Pesquisa e Desenvolvimento (INFOTECA-E) Palavras-chave: Classificação taxonômica; Taxonomic classification; Patogenicidade; Controle Biológico; Pathogenicity; Biological control.
Ano: 2019 URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/1111134
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Anti-Escherichia coli activity of Brazilian plant extracts: New trends in Veterinary research Arq. Bras. Med. Vet. Zootec.
Camargo,L.R.P.; Suffredini,I.B..
Escherichia coli é uma bactéria presente no trato intestinal de animais de sangue quente. Entretanto, a perda do equilíbrio em sua relação com o hospedeiro pode causar doenças que levam a perdas econômicas, quando se trata de animais de produção, e perdas incalculáveis, quando se trata de animais silvestres. O presente estudo testou diversos extratos aquosos e orgânicos, obtidos de plantas da Amazônia brasileira, contra Escherichia coli (ATCC 25922). Usando o ensaio de disco difusão em ágar Mueller-Hinton (DDA), foram testados 1.791 extratos quanto à sua atividade antibacteriana. Os extratos que mostraram halo de inibição de crescimento bacteriano foram testados no modelo da microdiluição em caldo (MDBC) contra uma suspensão bacteriana preparada na escala...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Escherichia coli; Plant extracts; Pathogenicity.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352014000200041
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Antimicrobial susceptibility and pathogenicity of Escherichia coli strains of environmental origin Ciência Rural
Carvalho,Daiane; Finkler,Fabrine; Grassotti,Tiela Trapp; Kunert Filho,Hiran Castagnino; Lima,Francisco Esmaile de Sales; Soares,Beatriz Dugaich; Rossato,Juliana Marzari; Cunha,Augusto César da; Brito,Kelly Cristina Tagliari de; Brito,Benito Guimarães de.
The study aimed to evaluate the antimicrobial susceptibility of 109 samples of Escherichia coli (E. coli) of environmental origin and to characterize these isolates according to the degree of pathogenicity in vivo, verifying a possible relationship between this variable and susceptibility to the active principles tested. The isolates were subjected to disc diffusion test to 14 antibiotics. From 16.5% to 90% of the samples were sensitive; 1 - 28.5% showed intermediate degree of susceptibility and between 9 to 78% of E. coli analyzed were resistant. The highest resistance percentages were seen in the class of quinolones and tetracyclines (>75%), and for sensitivity in the class of amphenicols (68.8%). By inoculating 1- day - old chicks, the isolates...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Escherichia coli; Resistance; Antimicrobials; Pathogenicity; Poultry..
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782015000701249
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Antracnosis del fruto de litchi (Litchi chinensis Soon.) : etiología y control in vitro. Colegio de Postgraduados
Martínez Bolaños, Misael.
El litchi (Litchi chinensis Soon) es un fruto subtropical. El cultivar Brewster se explota comercialmente en la región del Papaloapan y el cv. Mauritius en los Bajos Mixes, Oaxaca, México. El manchado del pericarpio es uno de los problemas principales en pre y postcosecha del fruto en ambos cultivares. No se han encontrado registros de su causa o importancia en México, por ello los objetivos de esta investigación fueron: determinar la etiología del manchado café del pericarpio e iniciar la exploración del control in vitro de su agente causal. La antracnosis o manchado del pericarpio del litchi se caracterizó externa e internamente por lesiones circulares de color café, con márgenes bien definidos en el pericarpio, pero sin afectar el arilo o la semilla....
Palavras-chave: Brewster; Mauritus; Pathogenicity; Incidence; PCR; Patogenicidad; Incidencia; Antracnosis; Benomil; Procloraz; Hidróxido de cobre; Doctorado; Fitopatología.
Ano: 2012 URL: http://hdl.handle.net/10521/1701
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Application of semi-selective mediums in routine diagnostic testing of Pseudomonas savastanoi pv. phaseolicola on common bean seeds Scientia Agricola
Popovic,Tatjana; Milovanovic,Predrag; Aleksic,Goran; Gavrilovic,Veljko; Starovic,Mira; Vasic,Mirjana; Balaž,Jelica.
Halo blight, caused by Pseudomonas savastanoi pv. phaseolicola (Psp), is considered to be an important bacterial disease on common bean (Phaseolus vulgaris L.) in Serbia. Use of pathogen-free seeds is one of the most effective control measures against this disease. The aim of this study was to evaluate a detection method for Psp on untreated common bean seeds (23 genotypes) from commercial crops grown within Serbia. Detection of this pathogen was made by plating onto the modified sucrose peptone (MSP) and Milk Tween (MT) semi-selective mediums from soaked whole common bean seed. Colonies growing on the MSP medium were light yellow, convex and shiny, whereas on the MT medium, they were creamy white, flat and circular. The pathogenicity of the obtained...
Tipo: Info:eu-repo/semantics/article Palavras-chave: MSP; MT; ELISA; Nested-PCR; Halo blight; Pathogenicity.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162012000400005
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Avaliação do potencial de patogenicidade e toxicidade do fungo entomopatógeno Colletotrichum gloeosporioides isolado de orthezia em duas espécies de crustáceos. Infoteca-e
JONSSON, C. M.; GENTHNER, F. J..
O micoinseticida Colletotriclum gloeosporioides isolado de Orthezia tem recebido especial atenção devido ao seu sucesso no controle de Orthezia praelonga em citros. Uma suspensão de esporos (10 6 esporos/ml) desse fungo, crescido em dois diferentes meios, foi avaliada quanto a sua capacidade de causar patogenicidade em embriões do camarão Palaemonetes pugio. Testaram-se o filtrado de meio cultura e o extrato orgânico seco do fungo com a finalidade de avaliar a toxicidade aguda no microcrustáceo Artemia salina. Os resultados demonstraram as ausência de efeitos adversos associados a capacidade do fungo em provocar infecção ou exercer efeitos tóxicos agudos.
Tipo: Folhetos Palavras-chave: Avaliacao de risco; Biopesticidas; Inseticida biologico; Orthezia praelonga; Colletotrichum gloeosporioides; Fungo para controle biológico; Artemia salina; Camarão; Toxidez; Controle biológico; Mycoinsecticides; Crustacea; Toxicity; Pathogenicity; Palaemonetes pugio.
Ano: 1997 URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/12999
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Bacterial disease in marine bivalves, a review of recent studies: Trends and evolution ArchiMer
Paillard, Christine; Leroux, Frederique; Borrego, Juan.
The main microbial diseases affecting marine cultured bivalves have been revised on the basis of the etiologic agents, pathogenesis and pathogenicity. Several recent bivalve-interaction models have been studied, including Pecten larvae-Vibrio pectinicida, brown ring disease, juvenile oyster disease, Pacific oyster nocardiosis and summer mortalities of oysters. In addition, the taxonomy and phylogeny of new potential bivalve pathogens and their virulence factors have been established. Facing the difficulty of identifying bacterial strains associated with molluscan diseases (mainly vibriosis), polyphasic approaches have been developed to correlate the phenotype and genotype of potential pathogens. By evaluating likely virulence mechanisms, developing...
Tipo: Text Palavras-chave: Diagnostic tools; Virulence factors; Pathogenicity; Pathogenesis; Etiology; Bivalve mollusc; Vibriosis.
Ano: 2004 URL: http://archimer.ifremer.fr/doc/2004/publication-407.pdf
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Botryosphaeriaceae from Eucalyptus plantations and adjacent plants in China Naturalis
Li, G.Q.; Liu, F.F.; Li, J.Q.; Liu, Q.L.; Chen, S.F..
The Botryosphaeriaceae is a species-rich family that includes pathogens of a wide variety of plants, including species of Eucalyptus. Recently, during disease surveys in China, diseased samples associated with species of Botryosphaeriaceae were collected from plantation Eucalyptus and other plants, including Cunninghamina lanceolata, Dimocarpus longan, Melastoma sanguineum and Phoenix hanceana, which were growing adjacent to Eucalyptus. In addition, few samples from Araucaria cunninghamii and Cedrus deodara in two gardens were also included in this study. Disease symptoms observed mainly included stem canker, shoot and twig blight. In this study, 105 isolates of Botryosphaeriaceae were collected from six provinces, of which 81 isolates were from Eucalyptus...
Tipo: Article / Letter to the editor Palavras-chave: Botryosphaeria; Cophinforma; Lasiodiplodia; Neofusicoccum; Pathogenicity; Plant pathogen.
Ano: 2018 URL: http://www.repository.naturalis.nl/record/639310
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Calonectria spp. causing leaf spot, crown and root rot of ornamental plants in Tunisia Naturalis
Lombard, L.; Polizzi, G.; Guarnaccia, V.; Vitale, A.; Crous, P.W..
Calonectria spp. are important pathogens of ornamental plants in nurseries, especially in the Northern Hemisphere. They are commonly associated with a wide range of disease symptoms of roots, leaves and shoots. During a recent survey in Tunisia, a number of Calonectria spp. were isolated from tissues of ornamental plants showing symptoms of leaf spot, crown and root rot. The aim of this study was to identify these Calonectria spp. using morphological and DNA sequence comparisons. Two previously undescribed Calonectria spp., C. pseudomexicana sp. nov. and C. tunisiana sp. nov., were recognised. Calonectria mexicana and C. polizzii are newly reported for the African continent. Pathogenicity tests with all four Calonectria spp. showed that they are able to...
Tipo: Article / Letter to the editor Palavras-chave: Calonectria; Crown and root rot; DNA phylogeny; Leaf spot; Pathogenicity; Systematics.
Ano: 2012 URL: http://www.repository.naturalis.nl/record/531828
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Caractérisation de la virulence d'une souche de Vibrio aestuarianus, pathogène de l'huître Crassostrea gigas ArchiMer
Labreuche, Yannick.
In France. shellfish production is a traditional. well-established industry. mainly based upon commercial farming of the Pacifie oyster. Crassostrea gigas. Since the 1980's, annual mass mortalities of C. gigas have been reported during summer. Several studies on this subject have demonstrated that these mortality outbreaks resulted from complex interactions between the physiologieal andJor genetic status of the oysters. environmental factors, and one or more infectious agents, among which are bacteria of the genus Vibrio.
Tipo: Text Palavras-chave: Huître; Crassostrea gigas; Bactéries; Vibrio aestuarlanus; Pathogénicité; Produits extracellulaires; Métalloprotéase; Hémocytes; Interactions hôte/pathogène; Oyster; Crassostrea gigas; Bacteria; Vibrio aestuarianus; Pathogenicity; Extracellular products; Metalloprotease; Hemocytes; Host/pathogen interactions.
Ano: 2006 URL: http://archimer.ifremer.fr/doc/00034/14561/11859.pdf
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Caracterización morfológica, molecular e histopatología de hongos ophiostomatoides asociados al pino de las alturas (Pinus hartwegii Lindl) Colegio de Postgraduados
Pérez Vera, Omar Alejandro.
Los descortezadores (Coleóptera: Curculionidae: Scolytinae) son vectores de especies de Ophiostoma spp. que causan el manchado azul de la madera y contribuyen a la muerte de árboles en coníferas y angiospermas. En México, Dendroctonus adjunctus es uno de las principales plagas de bosques de pino, Sin embargo, los hongos simbiontes que introduce en sus galerías y las alteraciones que pueden ocasionar en el sistema de conducción de agua del árbol se desconocen. Las especies del género Ophiostoma son difíciles de identificar por características morfológicas; por ello, recientemente se ha utilizado la técnica de reacción en cadena de la polimerasa (PCR) y secuencias de ADN. Por lo anterior, los objetivos de la presente investigación fueron: a)...
Tipo: Tesis Palavras-chave: Ascosporas; Secuencias ITS; Ciclohexamida; Patogenicidad; Doctorado; Fitopatología; Acospores; ITS sequences; Cycloheximide; Pathogenicity.
Ano: 2009 URL: http://hdl.handle.net/10521/1340
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Caracterización morfológica, molecular e histopatología de hongos ophiostomatoides asociados al pino de las alturas (Pinus hartwegii Lindl) Colegio de Postgraduados
Pérez Vera, Omar Alejandro.
Los descortezadores (Coleóptera: Curculionidae: Scolytinae) son vectores de especies de Ophiostoma spp. que causan el manchado azul de la madera y contribuyen a la muerte de árboles en coníferas y angiospermas. En México, Dendroctonus adjunctus es uno de las principales plagas de bosques de pino, Sin embargo, los hongos simbiontes que introduce en sus galerías y las alteraciones que pueden ocasionar en el sistema de conducción de agua del árbol se desconocen. Las especies del género Ophiostoma son difíciles de identificar por características morfológicas; por ello, recientemente se ha utilizado la técnica de reacción en cadena de la polimerasa (PCR) y secuencias de ADN. Por lo anterior, los objetivos de la presente investigación fueron: a)...
Tipo: Tesis Palavras-chave: Ascosporas; Secuencias ITS; Ciclohexamida; Patogenicidad acospores; ITS sequences; Cycloheximide; Pathogenicity.
Ano: 2012 URL: http://hdl.handle.net/10521/1043
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Cebos para el econtrol de la cucaracha alemana Blatella germanica (Dictyoptera: Blattellidae) formulados con hongos entomopatógenos y ácido bórico. Colegio de Postgraduados
Hernández Ramírez, Gabriela.
Blattella germanica (Dictyoptera: Blattellidae) es la especie más importante de las cucarachas sinantrópicas en el mundo. En este trabajo se evaluó la patogenicidad de aislamientos de Metarhizium anisopliae, M. pingshaense y Beauveria bassiana en ninfas y adultos de B. germanica. Para ofrecer una alternativa al uso de insecticidas en el manejo de B germanica, se desarrolló una formulación de cebo en polvo y pellet. Se determinó la preferencia de adultos de esta cucaracha, a diferentes dosis de azúcar glas (0.125, 0.25, 0.5, 0.75M) como fagoestimulante y se evaluó la compatibilidad del ácido bórico con los hongos M anisopliae y B bassiana en estos cebos. Finalmente, se evaluó vía oral, la virulencia de cebos formulados con azúcar glas 0.5 M, ácido bórico...
Palavras-chave: Cucaracha alemana; Hongo entomopatógeno; Plagas urbanas; Virulencia; German cockroach; Entomopathogenic fungi; Pathogenicity; Urban pest; Entomología y Acarología; Doctorado.
Ano: 2013 URL: http://hdl.handle.net/10521/1867
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Cgl-SLT2 is required for appressorium formation, sporulation and pathogenicity in Colletotrichum gloeosporioide BJM
Yong,H.Y.; Bakar,F.D.A.; Illias,R.M.; Mahadi,N.M.; Murad,A.M.A..
The mitogen-activated protein (MAP) kinase pathways has been implicated in the pathogenicity of various pathogenic fungi and plays important roles in regulating pathogenicity-related morphogenesis. This work describes the isolation and characterization of MAP kinase gene, Cgl-SLT2, from Colletotrichum gloeosporioides. A DNA sequence, including 1,633 bp of Cgl-SLT2 open-reading frame and its promoter and terminator regions, was isolated via DNA walking and cloned. To analyze gene function, a gene disruption cassette containing hygromycin-resistant gene was constructed, and Cgl-SLT2 was inactivated via gene deletion. Analysis on Cgl-slt2 mutant revealed a defect in vegetative growth and sporulation as compared to the wild-type strain. When grown under...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Colletotrichum gloeosporioides; MAP kinase; Pathogenicity; Appressorium.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000400031
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Characterization of abalone Haliotis tuberculata-Vibrio harveyi interactions in gill primary cultures ArchiMer
Pichon, Delphine; Cudennec, Benoit; Huchette, Sylvain; Djediat, Chakib; Renault, Tristan; Paillard, Christine; Auzoux-bordenave, Stephanie.
The decline of European abalone Haliotis tuberculata populations has been associated with various pathogens including bacteria of the genus Vibrio. Following the summer mortality outbreaks reported in France between 1998 and 2000, Vibrio harveyi strains were isolated from moribund abalones, allowing in vivo and in vitro studies on the interactions between abalone H. tuberculata and V. harveyi. This work reports the development of primary cell cultures from abalone gill tissue, a target tissue for bacterial colonisation, and their use for in vitro study of host cell—V. harveyi interactions. Gill cells originated from four-day-old explant primary cultures were successfully sub-cultured in multi-well plates and maintained in vitro for up to 24 days....
Tipo: Text Palavras-chave: Haliotis tuberculata; Vibrio harveyi; Gill cell culture; Pathogenicity; Phenoloxidase; Phagocytosis.
Ano: 2013 URL: http://archimer.ifremer.fr/doc/00146/25683/23745.pdf
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Clinical and pathological characterization of Blood stream forms and cerebrospinal fluid T. b. rhodesiense trypanosomes isolated from a patient using rabbits OAK
Ndungu, K; Kagira, J.M; Ngotho, J.M; Ouma, J; Bett, B; Gitonga, P.
Clinical and pathological characterisation of blood stream (BSF) and cerebrospinal fluid (CSF) forms of Trypanosoma brucei rhodesiense trypanosome isolated from a sleeping sickness patient were investigated in rabbits. The study aimed at investigating whether there is any significant difference in clinical and pathological presentation in rabbits infected by the two forms of trypanosomes. Each form of parasite was inoculated into five rabbits at 104 trypanosomes/ml while five rabbits were used as un-infected controls. Parasitaemia development, body temperature, packed cell volume (PCV), body weight, food and water intake, heartbeat and respiration were monitored daily for 30 days post infection when the experiment was terminated. Pathological changes were...
Palavras-chave: Pathogenicity; Rabbits; T. b. rhodesiense; CSF and Blood stream forms.
Ano: 2009 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2387
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